FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001592670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001592670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964998
Sequences flagged as poor quality0
Sequence length100
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTA25900.2683943386411164TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGATTCATCTCGTA24230.25108860329244204TruSeq Adapter, Index 25 (97% over 39bp)
CAGAGGCCGTGGCTGGTGTGGACGTGCTCCTGGTCTTTGAGGGGCATTCT14250.14766869983150224No Hit
CACGGGCGTGTCCAGCATCGCCAGCGAAGATAGCCGCAAGGTGGCATCTG14250.14766869983150224No Hit
CATGGAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGG13640.1413474432071362No Hit
CAGTACTTCTACTCTGACCTCCACAACCCGACGCGCATCACCCATGTCTA13020.13492255942499362No Hit
CATGGAGAGGAGGCCCCGCCCCACGCTGCCTCTGCTGTCAGCCCTGGTCA11920.12352357206957942No Hit
CATGGAGAGCAGCAGTGGGGAGGAGTACTATGTTGCCTCTGATAACACAG11150.11554428092078947No Hit
CTTGGTCGGGATCCCGGGCAAATACTACAGCCACAGGGGTCTTCACTGTG10950.11347173776525962No Hit
CTCCCTTGTCTCCCCACTGCGGCGGATCAAAGTAAGACGTTCCAAACATG10820.11212458471416521No Hit
TCAGGTGGCTGAAGGAGAGAATGTATTTGGTGTCTGCCATATCTTTGCAT10240.10611420956312863No Hit
CCCTGTTGTTCTGCATATCTTTGTTAGCCCCGTTCTTCAGGAGCACAACT10190.10559607377424617No Hit
CCAGGTCAGCTGGCACTGCAGGCTCTGGGACCGATGCGGCCTCCTGGATC10040.10404166640759878No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)