FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001592671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001592671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964998
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC89560.9280848250462695Illumina Single End PCR Primer 1 (100% over 50bp)
GCTGAGTGAGTGATGATGGATGCTAGCTCCTGAAACTCGTAGAAGGTTCC14810.15347182066698584No Hit
GCCAATCTTCACAAAGTAGTGGGTGTCCTTGCCCTCGATGCTGTAGTGCA14800.15336819350920936No Hit
GGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCGGCTC14040.14549252951819588No Hit
GCAAAGAGGTGGCCCTGGAGGTCGTAGTACAGTGAGGTAATCTCCGAGTT12990.13461167795166415No Hit
GTCCAGACTCCTCGCAAAATGTTTCAGGTCCTTCAGACTCCCACAGTGCA10990.11388624639636559No Hit
GGCGCCATCCATCAGAACATATCGCACTTCACCTGCGGGGCCCAGGTCAG10930.11326448344970662No Hit
GCATGTTCTCGTGTGCGTAGGTCGTCACCGCCGGAAAGGAGCGAAAGCGG10820.11212458471416521No Hit
GCCTTTTGCCAGCTGGATGCCTCTCCACCCAGGTACTCACTTGCCAGAAA10720.11108831313640027No Hit
GCGACGGATGGAGGTGGCCGATCGTGCCAGGCAGACATCACCCTCCATGT10280.1065287181942346No Hit
GTCTCCAACCTACCCCATCTGCTTCTTTCACGCAGGCAAGTCCGCCCTGC10250.10621783672090511No Hit
GTTCCCGAGGGGCTGTTCTCCTGCACCTGGCCCTGGTGCACGAAGGAGGC10100.10466342935425772No Hit
GCCTGATGGTGGGGCCACACGCTGCTGCGCGCAACCTCTGGGGCAACCTC9950.10310902198761034No Hit
CAGGCAGCAGTCAGGGTCCAAACAATCCACCAGGCCATCTGGAAGGGCAG9720.100725597358751No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)