FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001592676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001592676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1095963
Sequences flagged as poor quality0
Sequence length100
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTA25560.23321955211991646TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGATTCATCTCGTA17770.16214051021795445TruSeq Adapter, Index 25 (97% over 39bp)
CATGGAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGG14000.12774153871982905No Hit
CCAGGTCAGCTGGCACTGCAGGCTCTGGGACCGATGCGGCCTCCTGGATC13130.11980331452795395No Hit
AGCGGGATGGTCTGAAACCTCCTCCACCCTACAGAAAATATGATTGGATC11440.10438308592534602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)