FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001592680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001592680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008147
Sequences flagged as poor quality0
Sequence length100
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTA28190.27962192021600024TruSeq Adapter, Index 22 (97% over 38bp)
CAAGCGTGCTATATAAGCGTTGCTCAAGTCCCACCCCTTTCTTTTTGAGG19370.19213467877204415No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGATTCATCTCGTA16980.16842781856217398TruSeq Adapter, Index 25 (97% over 39bp)
TTGTGTGCTAGGGTGGCGGGTGAGGTGGGGCGGGTAGTGACCACACGTGT14690.14571287718953685No Hit
CATGGAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGG14100.13986055604986178No Hit
TGAGCAGTGACGAGAACAGCAATCCAGACCTCTCTGGAGACGAGAATGTA13870.13757914272422572No Hit
CACGGCCGCAGACACCTACAACATCACCGACCCGGAAGAGCTGGAGACAG13840.13728156707305583No Hit
CACGGGCGTGTCCAGCATCGCCAGCGAAGATAGCCGCAAGGTGGCATCTG12920.12815591377051164No Hit
TAAAACTCTCTCTCCCCCCCCACCCCGCTGCGCGTGCGCACACGTTACTG12100.12002217930520054No Hit
TGAGCAGCGCTTCCTTTTTGTCCGACATCTTGACGAGGCTGCGGTGTCTG12090.11992298742147722No Hit
CAGTACTTCTACTCTGACCTCCACAACCCGACGCGCATCACCCATGTCTA12060.1196254117703073No Hit
TCAGGTGGCTGAAGGAGAGAATGTATTTGGTGTCTGCCATATCTTTGCAT11930.11833591728190433No Hit
AGCGGGATGGTCTGAAACCTCCTCCACCCTACAGAAAATATGATTGGATC11840.11744319032839458No Hit
TCAGCCCAGTGGGCTTTAGGCCCCTGGGGTAGAGGGGCATTGTGAGGCCC11330.11238440425850596No Hit
TCCACCATGACTGAGCTGTGTTGCTCTTCACTCATTAGGATATCGTAAGC11150.11059895035148645No Hit
CATGGAGAGCAGCAGTGGGGAGGAGTACTATGTTGCCTCTGATAACACAG11140.11049975846776314No Hit
TAATCGTGAAGTGGAACCCTCCCTCTCTGCCCAACGGCAACCTGAGTTAC10550.10464743732808808No Hit
TGAGGGAAGGGATGCTAGGTGTGGAAGACAGGAACCAGGGCCTGATTAAC10460.1037547103745783No Hit
TAAGGACACACCTCCGATTTTGACTGTGAAATCTTCGGCTACCATGCAAT10360.10276279153734526No Hit
CATGGGAATGGTGAATGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATT10190.10107652951404905No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)