FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001592681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001592681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008147
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC81720.8105960737868585Illumina Single End PCR Primer 1 (100% over 50bp)
GCTGTTGCGCGCGGGCTTCCTGACCGACTTGTTCCTCGGCGAGAGCGAAC19660.19501124340002002No Hit
GCAGCGACTCCGAGGGGTTTGTGATCCACGACGGCGAGTGCATGCAGGAG17230.1709076156552566No Hit
GCCTGATGACGTCCGTCCTGGTCTGCCCTGATGGGAAGACGATTGAGGCT16050.15920297337590647No Hit
CCTTGCCGCTGCCCCAGTCTTCGACCTGCTGATCCTTGGATCCTGAATCT14990.14868863370123603No Hit
GGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCGGCTC14410.14293550444528427No Hit
GCCTCGTGGTTGCAGCTGTGGATATCGATGCGGTACAATGTGAAAGGCCG13800.1368847995381626No Hit
GTGACACCTCTCCCTCCCCCCACAGCAGCCCGCTCGTCCCCGCGCCCCCC13530.1342066186776333No Hit
GCCAATCTTCACAAAGTAGTGGGTGTCCTTGCCCTCGATGCTGTAGTGCA13390.13281793230550704No Hit
GCTGAGTGAGTGATGATGGATGCTAGCTCCTGAAACTCGTAGAAGGTTCC13010.1290486407240214No Hit
GCCTTTTGCCAGCTGGATGCCTCTCCACCCAGGTACTCACTTGCCAGAAA12470.12369227900296287No Hit
GTCCTCAAACCCAAGAACGCCGGCGACTTCACACCCCTGAAGCTTACCAT12270.12170844132849674No Hit
GCAAAGAGGTGGCCCTGGAGGTCGTAGTACAGTGAGGTAATCTCCGAGTT11750.11655046337488481No Hit
GGCCGCTCATGGGACCGTCACCCGCCACTATCGGGAGCACCAGAAGGTGA11590.11496339323531192No Hit
GGCAGATGGTTTCCCTGGGGACAAAAGCACAGGGTCATTTCTTCCTCGTC11320.11228521237478263No Hit
CCGCTGCTGCACCCTTACCTTTGGAGCAGTAATTGTGCCGGTAAAGGTAG10820.10732561818861733No Hit
GGCGCCATCCATCAGAACATATCGCACTTCACCTGCGGGGCCCAGGTCAG10760.10673046688627749No Hit
GCAAGATGATTCAGATGGCCGGAGAGATTGCAGACGGCATGGCATACCTC10650.10563935616532112No Hit
GCCCGACCTGTCTTCTTCTTCTTCTTCTCCTGTTTGGCCACCTGGAGAAG10440.10355632660713171No Hit
GTTCCCGAGGGGCTGTTCTCCTGCACCTGGCCCTGGTGCACGAAGGAGGC10390.10306036718851516No Hit
GCGCGGGCGAGTGTAGGGCTGGCGCTGCCGGACGCGGTGCTAGTCGCCGG10150.10067976197915582No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)