FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001592682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001592682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971854
Sequences flagged as poor quality0
Sequence length100
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTA27770.2857425086484184TruSeq Adapter, Index 22 (97% over 38bp)
AGCGGGATGGTCTGAAACCTCCTCCACCCTACAGAAAATATGATTGGATC20700.2129949560324905No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGATTCATCTCGTA18690.1923128371133936TruSeq Adapter, Index 25 (97% over 39bp)
TTGTGTGCTAGGGTGGCGGGTGAGGTGGGGCGGGTAGTGACCACACGTGT16850.17337995213272778No Hit
CAGTACTTCTACTCTGACCTCCACAACCCGACGCGCATCACCCATGTCTA15270.15712236611672123No Hit
TCAGACGGTAAAGTACCCATCCCGGGGTACCTGCAGTGGGGTGAAAGGGC13750.14148215678486686No Hit
CATGGAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGG12380.12738538916339284No Hit
CAGGTTGGAGGATGGTTGGTTGTGGCGAGCGAGGCTGAAGGAGCCGGGAC12060.1240927135145814No Hit
CATGGAGAGGAGGCCCCGCCCCACGCTGCCTCTGCTGTCAGCCCTGGTCA11750.12090293397979532No Hit
CACGGGCGTGTCCAGCATCGCCAGCGAAGATAGCCGCAAGGTGGCATCTG11500.11833053112916138No Hit
TCCACCATGACTGAGCTGTGTTGCTCTTCACTCATTAGGATATCGTAAGC11370.11699288164683172No Hit
CCCACTTCGGTACTCCCCCTCCTTCCCAGGTCCTCCAGCCCTTCTGCGAC11310.11637550496267958No Hit
CGTGGATAATGTGCACCATAACCCTCCCACCATTGAACTGTTGCACCGCT11030.11349441376996955No Hit
CGCCGCTTCGTCTGCACCAGCAAGGACTATGGGCCAAGAGGGGAAGGGAT10980.11297993319984279No Hit
CTTGGATCTCATTGAAACACTTCTCACAGAAATGATACCTGTCGGCAAGA10780.11092201091933561No Hit
TGAGGGAAGGGATGCTAGGTGTGGAAGACAGGAACCAGGGCCTGATTAAC10600.10906988086687919No Hit
CGTGGGGCTGTGAGTACCTTGCTGCAGGCCCCAGAGCTGCCCACCAAGAC10200.10495403630586488No Hit
TCAGGCCTGTGGGATCCCATCATCCCCATCCCTGCTCACACGTTTACCTG9840.10124977620095199No Hit
TGAGATTTAGTGACTTCTTTAGGGAAGAATAATACACACATGGGGACCAG9780.10063239951679985No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)