FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001592683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001592683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971854
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC84640.8709127091106278Illumina Single End PCR Primer 1 (100% over 50bp)
GCAGCGACTCCGAGGGGTTTGTGATCCACGACGGCGAGTGCATGCAGGAG19750.2032198252000815No Hit
GCAAAGAGGTGGCCCTGGAGGTCGTAGTACAGTGAGGTAATCTCCGAGTT14890.15321231378375766No Hit
GGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCGGCTC13220.1360286627415229No Hit
TCTTACCCTCTCATCCCCCTCAGATGCATCTGAAGAAACATCGCTGGTAT12730.13098675315428038No Hit
GCTGAGTGAGTGATGATGGATGCTAGCTCCTGAAACTCGTAGAAGGTTCC12390.1274882852774182No Hit
GCTGCGGACAGCGACGGGGAGAAGGGGCGGAGGTGAGTACCGGCTCAGTT12310.12666511636521535No Hit
GGACAGGCTGGTCTGTGAACTTTCACCAGGAAGTGAACCGAATGACCCAG12290.12645932413716465No Hit
GCCAATCTTCACAAAGTAGTGGGTGTCCTTGCCCTCGATGCTGTAGTGCA12060.1240927135145814No Hit
CCTTGCCGCTGCCCCAGTCTTCGACCTGCTGATCCTTGGATCCTGAATCT11910.12254927180420105No Hit
GGCAGATGGTTTCTCTGGGGACAAAAGCACAGGGTCATTTCTTCCTCGTC11560.11894790781331352No Hit
TGCTCTCCGGCCGGGCCAACGCGCGGGGCCTGCTTGATGCTAATGTCTAC11480.11812473890111067No Hit
GCCTGATGACGTCCGTCCTGGTCTGCCCTGATGGGAAGACGATTGAGGCT10960.11277414097179206No Hit
GATCGTGTGGCTTCCCAGATGCAGGGGCGCTGTGAACAGTGCTGAGGAAA10890.11205386817361455No Hit
GCCCGACCTGTCTTCTTCTTCTTCTTCTCCTGTTTGGCCACCTGGAGAAG10790.11102490703336099No Hit
GCCTGCTGTGATTGGTGGCTTCGTTGCTTGGCTTGGGCTGTGTTGTGTGA10720.11030463423518347No Hit
GCCGGGGAGAGGCGGCGGATCATGTTGTCCAGGGGGGACCGGAGGGGCCG10580.10886408863882847No Hit
GGCACGTCAGTGGTGTCTCTGTGCTCTGCAGCTCTCCTGGAGGTCCTCGT10470.10773223138454953No Hit
GCCTGCTGATAGAGCTGCTCCTTGGTGAGGGGCACAGGGCCCAGTGGACA10430.1073206469284481No Hit
GTCCAGACTCCTCGCAAAATGTTTCAGGTCCTTCAGACTCCCACAGTGCA10020.10310190625340843No Hit
GGGTGGAGTAGCTGAATGCCGCTGCTAGTTATAAATTGGGCTATATTTTC9870.10155846454302808No Hit
GCTGCTCGCGTCAGTCTGTCTCCACTCTCCCCAACACAGATGTTGAGACA9850.10135267231497737No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)