FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001592696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001592696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868331
Sequences flagged as poor quality0
Sequence length100
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTA21110.24311005826119303TruSeq Adapter, Index 22 (97% over 38bp)
CAGAGGCCGTGGCTGGTGTGGACGTGCTCCTGGTCTTTGAGGGGCATTCT9690.1115933900782075No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGATTCATCTCGTA9600.11055691896293003TruSeq Adapter, Index 25 (97% over 39bp)
CAGTACTTCTACTCTGACCTCCACAACCCGACGCGCATCACCCATGTCTA9080.10456841918577131No Hit
CTTGGCACCTGCTTTCCTCCTCCAGAAGAGAAGAATCCCTACAAAGAAGT9030.10399260189950606No Hit
CATGGAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGG8980.1034167846132408No Hit
CTTGGATCTCATTGAAACACTTCTCACAGAAATGATACCTGTCGGCAAGA8960.10318645769873469No Hit
CATCACAGAACACAACAGGCTGAAGGTTCCAGAGGACCTGCCCCCCGGGA8910.10261064041246944No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)