FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001592702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001592702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1231657
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTA36220.29407537975264214TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGATTCATCTCGTA21100.17131392912150054TruSeq Adapter, Index 25 (97% over 39bp)
CGTGGTCCACATTCCTGACCAGGTGTCTTTCTCACCCACTGGTTTCAACA13890.11277490405202098No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)