FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001592704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001592704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6897
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTA3765.451645643033203TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGATTCATCTCGTA1001.4499057561258517TruSeq Adapter, Index 25 (97% over 39bp)
TGCTTCAATATCTGGTTGAACGGCGTCGCGTCGTAACCCAGCTTGGTAAG450.6524575902566333No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGATGAATCTCGTA390.5654632448890822TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATCTCGTA380.5509641873278237TruSeq Adapter, Index 13 (97% over 38bp)
TCTGAATGCAATGAAGAAAACCACCATTACCAGCATTAACCGTCAAACTA350.5074670146440481No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTA220.3189792663476874TruSeq Adapter, Index 7 (97% over 36bp)
TGAGAGGAAGGACTTGAAAGAGCCACTGTATCTGCATCATCAGCTGAAAG180.26098303610265333No Hit
TGAGAAGGCAGTGACCAAGGAGGAATTTCAGGGTGAATGGACTGCTCCCG170.2464839785413948No Hit
AATTTTCCCCCAAATAGAACCTACCAAGAGATCGAGCAATCAAAGCGTTA130.18848774829636072No Hit
TTAGACACCGCCACCGACTCGGACATCGGCTCAAACGGTGTGGACCACCG120.17398869073510223No Hit
TAATAGCAATGACTCTCTCTGTTGAATTTTCCCGATCCAGTTTCTGCAGA120.17398869073510223No Hit
CCGAGGCAGGTGGATCACCTGAGGTCAGGAGTTCAAGACCAGCCTGGAGA120.17398869073510223No Hit
TCAGAAATGGCACCTCGAAAGGGGAAGGAAAAGAAGGAAGAACAGGTCAT110.1594896331738437No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTAGTAATCTCGTA110.1594896331738437TruSeq Adapter, Index 11 (97% over 38bp)
GGACGTCAATAGTCACACAGTCCTTGACGGTATAATAACCACCATCATGG100.1449905756125852No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTA100.1449905756125852TruSeq Adapter, Index 23 (97% over 37bp)
CTCCTCAGCCAGGTCCACGGGCAGACGGCCCCAGGCATCGCGCACGTCCA90.13049151805132667No Hit
AATTAAATGAACTAAATAAAGTGTTACCACTAATAAGTGTGACATACTAA80.11599246049006816No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAATGAATCTCGTA80.11599246049006816TruSeq Adapter, Index 1 (97% over 37bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTA80.11599246049006816TruSeq Adapter, Index 22 (97% over 38bp)
AATGGATATTCCAATGCTCAGAACTGACATCTGCACAGTGCGGGATGGTC80.11599246049006816No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAGAATCTCGTA70.10149340292880964TruSeq Adapter, Index 16 (97% over 37bp)
CCAAACCGAAAGTGCAATCAAATTCCCTCGGACTCGGTTGGCATTCCCCC70.10149340292880964No Hit
CAGCACACTGCTGCCTCACCATTGTCATCGCACGATTCGGACTGGAAGGA70.10149340292880964No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATATCGTA70.10149340292880964TruSeq Adapter, Index 22 (97% over 38bp)
TGCAGAGGTTCCTCAGCCATAAGCTGGACATCAAACGGGCTAACATTCAC70.10149340292880964No Hit
CCGAGGCAGGTGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGAGA70.10149340292880964No Hit
CATGGAGAGGAGGCCCCGCCCCACGCTGCCTCTGCTGTCAGCCCTGGTCA70.10149340292880964No Hit
CGAAAGGTCGCAAAGTAAGAGCTTCTCGAGCTGCGCAAGGATAGGTCGAA70.10149340292880964No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)