FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001592708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001592708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2916298
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTA88430.30322689930864405TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGATTCATCTCGTA41870.14357243326985103TruSeq Adapter, Index 25 (97% over 39bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)