FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001595833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001595833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23941888
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGC6332522.644954316050597No Hit
TGACAC5791302.418898626541065No Hit
ATACCT5717162.3879319792992098No Hit
CATGAG5651332.36043623627343No Hit
TTCCAT5606042.3415195994568183No Hit
GCCATA5420952.2642115776333096No Hit
CCACTT5250882.1931770794350056No Hit
GGCTAC5227412.183374176673118No Hit
TCCAGA5081822.1225644360210856No Hit
CTTCAC5080592.122050692075746No Hit
GCTCAA5049352.109002431220128No Hit
ACAGTG4991632.084894056809555No Hit
AGTGCA4933322.060539252376421No Hit
GCGGCT4587971.9162941535771951No Hit
ATTCGA4523341.8892996241566244No Hit
GTCTGC4514411.8855697595778578No Hit
GCCAAT4511511.8843584933652684No Hit
TCCTCC4496381.8780390251595862No Hit
AGGTGT4487011.8741253822589097No Hit
TTCACA4307141.7989976396180618No Hit
AGAACG4052361.6925816376720166No Hit
GACCAT4038821.6869262774932372No Hit
GCGTAT3621411.5125833017011858No Hit
GATTCA3454321.4427934839558183No Hit
TCTCGC3191841.333161361376346No Hit
TACTCG3024121.2631084064882436No Hit
GGCAGG2847771.1894508904226768No Hit
AATTGC2594021.0834650968211028No Hit
CTAGTT2567021.072187790703891No Hit
TGACCA2382490.9951136685628134No Hit
TGCGAC2364430.9875704038044117No Hit
AACATA2298680.9601080750189793No Hit
TAGCTT2133140.8909656581803406No Hit
TCATGT2125200.8876492948258717No Hit
CCGGAA2090430.873126630614929No Hit
ATTGAA2048240.8555047956117747No Hit
TCGGAC2048030.855417083230863No Hit
GTACTT1922900.8031530345476514No Hit
ACAAGT1740300.7268850309549523No Hit
TCTTCA1734250.7243580790286881No Hit
CAAGGA1705830.7124876701453118No Hit
ATCAGC1703110.7113515859735038No Hit
ACTTGA1696630.708645032505373No Hit
AGTTAG1669560.6973384889278573No Hit
CTGTAT1642150.6858899348288656No Hit
TGTGAT1614250.6742367185077467No Hit
CGATGT1573530.657228870170974No Hit
GAACCG1542090.6440970737144873No Hit
GATCAG1528630.6384751277760551No Hit
AGTTCC1501300.6270599879174107No Hit
TATAAG1492370.623330123338644No Hit
GTTGGC1470120.6140367877420527No Hit
GACTTG1447780.6047058611250709No Hit
GGCCGT1427670.5963063564577697No Hit
TGATAA1397180.5835713541054073No Hit
TACAGT1390030.5805849563743678No Hit
CAGTCC1381320.5769469809565562No Hit
GACTAA1360180.5681172679447837No Hit
AGGTCA1355670.5662335401452049No Hit
GTCTCA1281200.5351290591619173No Hit
TTCTAC1247080.5208778856537963No Hit
ATTCAT1227340.5126329218481015No Hit
CTACCG1209450.5051606623504379No Hit
CGTACT1192300.49799748457598664No Hit
CAGATC1183760.494430514418913No Hit
TTAGCG1171100.48914271088395367No Hit
TTCTGT1157270.4833662240839152No Hit
CCGCTC1155800.48275223741753365No Hit
GACACG1148120.47954447034419345No Hit
AACTCT1114650.4655647875388942No Hit
CGTCTC1110450.46381053992066124No Hit
CGACAA1108890.46315896223388897No Hit
TACGAA1098460.4588025806486105No Hit
AAGCGC1078270.45036966174096216No Hit
CCTGAC1074090.448623767682816No Hit
CCGTCG1066450.4454327077296494No Hit
ACCAGG1063230.44408778455567083No Hit
CGCTTG1042110.4352664251039851No Hit
TGCGGA1018940.42558882574340007No Hit
ATTGCT988920.41305013205307783No Hit
ACTAAT973850.40675572452765635No Hit
CCAGCG958720.40043625632197427No Hit
GGTAAT950490.3969987663462464No Hit
CTTGTA937760.39168172535098317No Hit
GTGGTG932000.38927590004597795No Hit
CTTAGC912320.3810559969205436No Hit
ATGTGA903580.3774054911626017No Hit
TTAGGC883490.3690143400553874No Hit
GCGCAG870820.3637223597403847No Hit
GCTGTC846110.3534015362531142No Hit
CTCCGC844050.3525411195641714No Hit
CGAAGC802080.33501117372197214No Hit
CTGAGG796670.33275153571848637No Hit
AGAGGT777350.32468199667461484No Hit
GCCTGG756620.31602353164462216No Hit
GTCGAG637000.26606088876533046No Hit
ATACTT248550.10381386797899983No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)