FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001595834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001595834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7479275
Sequences flagged as poor quality0
Sequence length6
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAC1868952.498838456936No Hit
CCACTT1851822.475935167512894No Hit
GCCATA1823532.438110645751092No Hit
GCTCAA1806642.4155282430449474No Hit
AGTGCA1770612.367355124661147No Hit
GTCTGC1759942.3530890360362466No Hit
ATACCT1749912.3396786453232434No Hit
TTCCAT1736352.3215485458149354No Hit
TTCACA1668502.230831196874029No Hit
TCCAGA1655482.2134230924788834No Hit
GCCAAT1617672.1628700642776204No Hit
ATTCGA1560142.0859508441660455No Hit
GACCAT1542452.06229881907003No Hit
AGAACG1447431.9352544197131407No Hit
GATTCA1417491.8952238017722305No Hit
ACAGTG1406981.8811716376253045No Hit
TACTCG1386391.8536422313660081No Hit
AGGTGT1379801.8448312169294483No Hit
TGCGAC1309581.7509451116585497No Hit
TCTCGC1283911.7166236032235744No Hit
TCATGT1279141.7102459797239704No Hit
CTAGTT1278971.710018684966123No Hit
AATTGC1238371.6557353486801862No Hit
ATTGAA1194211.5966921927593252No Hit
TCGGAC1190631.5919056325646534No Hit
TAGCTT1171271.5660207707297833No Hit
ACAAGT1170621.5651517025380133No Hit
TGACCA1125161.5043704102336122No Hit
TCTTCA1006311.34546463393845No Hit
CCGGAA986651.3191786637073781No Hit
ATCAGC982921.3141915493146061No Hit
AGTTAG979501.3096189135979088No Hit
TGTGAT956371.278693456250773No Hit
GGCCGT950071.2702701799305414No Hit
GACTTG928801.2418315946398548No Hit
GATCAG900371.2038198889598255No Hit
GAACCG890231.1902624251682148No Hit
CGACAA812701.086602645309873No Hit
CTGTAT812161.0858806501967102No Hit
TATAAG791481.0582309114185533No Hit
CTACCG721030.9640372897105669No Hit
TACGAA701480.9378983925581021No Hit
CTTGTA699420.9351441149041853No Hit
CGATGT691630.9247286668828194No Hit
ACTTGA687700.9194741468925799No Hit
TTCTGT670300.8962098599128927No Hit
ATGTGA653510.8737611600054818No Hit
CGCTTG632530.8457103128311234No Hit
AAGCGC628690.8405761253597441No Hit
AGGTCA628440.8402418683629095No Hit
ATTCAT626210.8372602959511449No Hit
TGATAA611870.8180873146127131No Hit
CTCCGC603080.806334838604009No Hit
CGTCTC584380.7813324152407821No Hit
ACTAAT573540.7668390318580344No Hit
TTCTAC568540.7601538919213426No Hit
TTAGGC565840.7565439163555291No Hit
GTGGTG553180.7396171420358257No Hit
CGTACT545860.729830097168509No Hit
CGAAGC525970.7032366105003492No Hit
CAGTCC521040.6966450625227713No Hit
ATTGCT507510.6785550738540835No Hit
CAGATC503260.6728727049078955No Hit
CCGTCG500530.6692226185024618No Hit
GGTAAT495980.6631391411600723No Hit
GCGCAG455290.608735472355275No Hit
GCCTGG419750.5612174976852703No Hit
CCAGCG356010.47599533377232417No Hit
CTTAGC346680.4635208626504574No Hit
AGAGGT310340.41493326559058197No Hit
CTGAGG308820.4129009830498277No Hit
GTCGAG240190.32114075227879707No Hit
ATTAAA178470.23861938490027443No Hit
AGCCGT98360.13151007283459962No Hit
AAAAAA90140.12051970277867842No Hit
GACTTA76740.1026035277483446No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)