FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001595835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001595835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32159086
Sequences flagged as poor quality0
Sequence length6
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGAA17170025.339088306178851No Hit
TTCTGT16898095.254530554755195No Hit
TTAGGC16318275.074233142073751No Hit
GAACCG11734943.6490278361766872No Hit
ATCAGC10908553.39205846832836No Hit
ACAAGT10566273.2856250951908272No Hit
TATAAG9611292.9886701381998235No Hit
ATACCT9473722.945892181139725No Hit
TCGGAC9226092.868890614615104No Hit
CTAGTT8888002.763760139202961No Hit
TTCCAT8152462.535040952345474No Hit
AACCTT7988172.483954301437547No Hit
CGAGCC7646642.377754143883318No Hit
CGATGT7603842.3644453079294605No Hit
TGACCA7413492.3052551928870115No Hit
CGACAA7073422.199509028334947No Hit
ACAGTG6732772.093582510398461No Hit
ATCCGG4993931.5528830639029978No Hit
AGTGCA4974121.5467230629626725No Hit
AAGCAT4900241.5237497732367145No Hit
GCCAAT4632781.440581986689547No Hit
ACACGA4607461.4327086285972184No Hit
TGCATA4330201.3464934917615508No Hit
TATTGG4262421.3254170221131285No Hit
CAGGAC4216511.3111411188738387No Hit
TAAGTC4073021.2665223134761978No Hit
GATCCT3912561.2166266168136743No Hit
GACCAT3610041.1225567791323423No Hit
ACCGCT2506530.7794158080238972No Hit
ATACAT2237000.6956043464668119No Hit
ATCCAT1900400.5909371926801651No Hit
GGAGGC1615690.5024054477170153No Hit
AGTACA1467360.45628162442178866No Hit
ACCAAT1398930.43500303460116996No Hit
AATCGG1368590.4255686868712624No Hit
AACCAT1119690.34817220862558096No Hit
TTACAT1053040.3274471171226695No Hit
TCTCTG1024000.3184170097371548No Hit
CTAGAT970440.3017623075481685No Hit
ATACCA947940.2947658400490611No Hit
TATTCT900200.2799208907865105No Hit
ATAGTT888130.2761676746658782No Hit
ACAGTA887460.2759593354114604No Hit
GAAGAT883220.2746408899805175No Hit
ATAACT823590.2560986963373275No Hit
ACAAAT792350.2463844899074557No Hit
ACGGAC779280.24232031967575196No Hit
ATAAAT777860.24187876483803056No Hit
GCAAAT749550.23307565395359808No Hit
GGACTA742910.2310109186560837No Hit
ACAATG721590.2243813770080406No Hit
CGTCAG713760.2219466063183512No Hit
TAACCA703410.2187282312687618No Hit
AGACCA675970.21019565046096148No Hit
ATACAA642450.19977246865784679No Hit
TTCCAA641500.19947706225232892No Hit
ACGAAC633390.19695522441153956No Hit
AAACAT632320.19662250351269311No Hit
CATACG614960.1912243401444929No Hit
GACAAT606170.18849105350817497No Hit
TCGAAC603820.18776031134715707No Hit
TTCAAT603690.18771988731271777No Hit
GGTACC589320.18325147673662118No Hit
ATCAAT557720.17342532682676368No Hit
CGTGAA555990.17288737621461006No Hit
GTAAGA554650.17247069770577433No Hit
CACATT553080.17198249975139218No Hit
ACAGTT550570.17120200493260287No Hit
AACAAT519870.16165571372270965No Hit
CTAATT516640.16065133194394893No Hit
AAGGCG510990.15889444121639526No Hit
GAGAAG509200.1583378333575774No Hit
AATGCA505680.15724327488660592No Hit
TCGGAA497690.15475875153914512No Hit
TTACCA496430.1543669493591951No Hit
AGTGAA490640.15256652505609145No Hit
AAAAAA481650.14977104759755921No Hit
CTGTTC477120.1483624254744056No Hit
GCCAAA473490.14723366205121627No Hit
TTGAAT466870.1451751458359233No Hit
TGACAA464460.14442574642824116No Hit
TTACGT459980.1430326720106411No Hit
GAACAT453830.14112030422755176No Hit
CTACCG452740.14078136424648385No Hit
ACAGAG440940.13711210573584087No Hit
CGAATT425980.13246023223421213No Hit
TGAACA424260.13192539116316926No Hit
AATACA422020.13122885395436923No Hit
TCCACG415970.12934758158238702No Hit
TCAGAC408740.1270993833593405No Hit
TCAAGC408340.1269750017149119No Hit
CAGCCT390620.121464894866726No Hit
ACAAAA389950.12125655561230812No Hit
AAACCT380200.11822475302936158No Hit
AAACAA369880.11501570660310433No Hit
TTACAA359560.11180666017684705No Hit
GACCAA357890.11128736681135776No Hit
AGAGCA353060.1097854584548827No Hit
GAGCCA350580.10901429225942552No Hit
ATAGAT350150.10888058199166482No Hit
TTCTAT348210.10827733101618621No Hit
AAAAAT344500.10712369126411117No Hit
CTGGAG341620.10622814342422543No Hit
AGTAAA340560.1058985320664897No Hit
CTATTT339750.10564665923652183No Hit
ATAATT332710.10345754229457889No Hit
AAACCA330730.10284185315465745No Hit
AATAAA326500.10152651726482524No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)