FastQCFastQC Report
Wed 27 Sep 2023
EGAF00001596816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001596816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34567069
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTA838410.24254587509285208TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT475370.13752106086865507TruSeq Adapter, Index 20 (97% over 44bp)
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT461710.13356932287200862No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC399980.11571128579053087No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC393420.11381352581556742No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG364770.10552529055905782No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)