FastQCFastQC Report
Sun 2 Apr 2023
EGAF00001596824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001596824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26963486
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA797350.2957147306546342TruSeq Adapter, Index 16 (97% over 40bp)
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT479660.17789242830099936No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC354240.13137767126995376No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG338580.1255698168997881No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC302400.11215167059630197No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT299330.1110130937817165TruSeq Adapter, Index 16 (97% over 40bp)
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACT299060.11091295836154125No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA282350.10471568846847176No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)