FastQCFastQC Report
Thu 16 May 2024
EGAF00001596832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001596832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41141597
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT1729240.4203142624726017TruSeq Adapter, Index 21 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTA878510.21353327630913307TruSeq Adapter, Index 21 (97% over 40bp)
GTTGATTATTCTCAACCAATCACAAAGATATCGGAACCCTCTATCTACTA621540.15107337714673547No Hit
CGTGGGAATGCTATATCTGGGGCTCCGATTATTAGTGGGACAAGTCAGTT619200.15050460972625831No Hit
ACTTGGTCTACAAGACGCCACATCCCCTATTATAGAAGAGCTAATAAATT567610.13796498954573883No Hit
TGGGAATGCTATATCTGGGGCTCCGATTATTAGTGGGACAAGTCAGTTTC521250.12669658885628576No Hit
TAATCGTTGATTATTCTCAACCAATCACAAAGATATCGGAACCCTCTATC479630.11658030678779922No Hit
CGGGGTTGTTGATTTCGTCTATTATATATAGAATGCGTAGAGAGGGGAGA440630.1071008497798469No Hit
GTGGGAATGCTATATCTGGGGCTCCGATTATTAGTGGGACAAGTCAGTTT425160.10334066516669248No Hit
TGATTATTCTCAACCAATCACAAAGATATCGGAACCCTCTATCTACTATT425070.10331878949667414No Hit
CTACAAGACGCCACATCCCCTATTATAGAAGAGCTAATAAATTTCCATGA412780.10033154522416812No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)