FastQCFastQC Report
Mon 3 Apr 2023
EGAF00001596833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001596833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41141597
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC863870.2099748339861479Illumina Single End PCR Primer 1 (100% over 50bp)
CGTGGGAATGCTATATCTGGGGCTCCGATTATTAGTGGGACAAGTCAGTT662870.16111917094516287No Hit
TGGGAATGCTATATCTGGGGCTCCGATTATTAGTGGGACAAGTCAGTTTC561120.13638751067441549No Hit
GTTGATTATTCTCAACCAATCACAAAGATATCGGAACCCTCTATCTACTA556770.1353301866235285No Hit
ACTTGGTCTACAAGACGCCACATCCCCTATTATAGAAGAGCTAATAAATT535890.13025503117927095No Hit
TAATCGTTGATTATTCTCAACCAATCACAAAGATATCGGAACCCTCTATC460510.11193294222390056No Hit
CCTAGGTTTAAATTAATTAATGGGTGTGGTATTGGTAGGGGAACTCATAG433360.10533378176836451No Hit
CGGGGTTGTTGATTTCGTCTATTATATATAGAATGCGTAGAGAGGGGAGA426560.10368095336697794No Hit
GTGGGAATGCTATATCTGGGGCTCCGATTATTAGTGGGACAAGTCAGTTT420210.10213750331568316No Hit
CAAGACGCCACATCCCCTATTATAGAAGAGCTAATAAATTTCCATGATCA417780.10154686022518766No Hit

[OK]Adapter Content

Adapter graph