FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001601867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001601867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439402
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG607924.223420559371183Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA604464.199382799245798Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT489133.3981472861646713Clontech SMART CDS Primer II A (100% over 26bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG211061.4663033676485095Clontech SMART CDS Primer II A (96% over 26bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA189451.316171576807591Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA163151.1334568105365979Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT147571.0252174166772035Clontech SMART CDS Primer II A (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA139720.9706808799765457Clontech SMART CDS Primer II A (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG134390.9336516136562266Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC133420.9269127040256996Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA130320.9053759825260768TruSeq Adapter, Index 7 (97% over 37bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC116330.808182842597134Clontech Universal Primer Mix Long (96% over 25bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC115790.8044312846584901Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC111750.7763640734138205Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC107270.7452400371821075Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCT105030.7296780190662511Clontech SMART CDS Primer II A (96% over 25bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA103800.7211328037615621Clontech Universal Primer Mix Long (96% over 25bp)
CTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTC103410.7184233452503191Clontech SMART CDS Primer II A (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT96520.6705562448850286Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC90560.6291501609696248Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATT88800.6169228610214519TruSeq Adapter, Index 7 (97% over 38bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC88500.6148386621666497Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG85750.5957335059976296Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC83170.5778093958463306Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA78680.5466158863194577TruSeq Adapter, Index 7 (97% over 35bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA76970.5347359528470851Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG75200.5224391796037521Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG74710.5190349881409085Clontech Universal Primer Mix Long (96% over 25bp)
ACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCG71030.4934688155220015Clontech SMART CDS Primer II A (96% over 26bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG67110.4662352838192527Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG66560.4624142525854487Clontech SMART CDS Primer II A (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC63400.4404606913148655Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCT63170.4388628055261838Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC60860.4228144743442068Clontech SMART CDS Primer II A (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT60850.4227450010490468Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG60260.4186460766346024Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA58740.4080861357702713Clontech SMART CDS Primer II A (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC58660.4075303494089907Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC57390.3987072409236614Clontech Universal Primer Mix Long (96% over 26bp)
TTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGAT56910.39537252275597784Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT56850.3949556829850174Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC55340.3844652154158463Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA53260.370014770022551Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA52890.36744425810162834Clontech Universal Primer Mix Long (96% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC52160.362372707554943Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT51100.3550085382679752Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA50820.3530632860034931Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG45980.31943821114601756Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA43020.2988741157786359Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA42670.29644255044803325Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG37190.25837118470031306Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG35820.24885334326338301Clontech Universal Primer Mix Long (96% over 25bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC35450.2462828313424603Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT35170.2443375790779782Clontech Universal Primer Mix Long (96% over 26bp)
AGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC34180.23745972285713093Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT32180.22356506382511626Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG31460.21856298657359097Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG31370.21793772691715033TruSeq Adapter, Index 7 (97% over 36bp)
TGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCAC31100.21606194794782835Clontech SMART CDS Primer II A (96% over 26bp)
TATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT30780.213838802502706TruSeq Adapter, Index 7 (97% over 38bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG30640.21286617637046495Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG29090.20209781562065357Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT28920.2009167696029323Clontech Universal Primer Mix Long (96% over 25bp)
ATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG28600.19869362415781TruSeq Adapter, Index 7 (97% over 36bp)
ATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT28580.19855467756748985TruSeq Adapter, Index 7 (97% over 38bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA28510.1980683645013693Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG28370.1970957383691283Clontech Universal Primer Mix Long (95% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG28190.19584521905624697Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT27930.19403891338208504Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT27310.18973156908216052Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG26660.18521580489675574Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA25520.17729584924850736Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG25290.1756979634598257Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC25230.17528112368886523Clontech SMART CDS Primer II A (96% over 26bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC24980.1735442913098634Clontech Universal Primer Mix Long (96% over 26bp)
TGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA24270.16861168735349819Clontech Universal Primer Mix Long (96% over 26bp)
AGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT24250.16847274076317803Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA23130.1606917317052498Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC22270.1547170283214835Clontech SMART CDS Primer II A (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT22060.15325808912312197Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC21680.15061810390703917Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT21180.1471444391490355Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT21120.14672759937807506Clontech SMART CDS Primer II A (96% over 26bp)
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA20190.14026658292818822Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG19690.13679291817018455Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTTCCATGTACTCTGCGTTGATACCACTGCTTTCCA19600.1361676585137439Clontech Universal Primer Mix Long (96% over 26bp)
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG19530.1356813454476234Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGTAAGCAGTGGTATCAACGCAGA19480.135333978971823Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC19260.1338055664783014Clontech SMART CDS Primer II A (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT19090.13262452046058015Clontech Universal Primer Mix Long (96% over 26bp)
TCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT18920.1314434744428589Clontech SMART CDS Primer II A (96% over 26bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA18870.13109610796705856Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA18540.12880348922677612Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG17850.12400983186073104Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA17810.12373193868009076Clontech SMART CDS Primer II A (96% over 26bp)
ATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAGAGTACATGGA17670.12275931254784973Clontech Universal Primer Mix Long (96% over 26bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT17320.12032774721724716Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA17320.12032774721724716Clontech SMART CDS Primer II A (96% over 26bp)
AACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCA17110.11886880801888562Clontech Universal Primer Mix Long (96% over 25bp)
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG16510.11470041030928121Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTTCCATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGC16360.11365831088188012Clontech Universal Primer Mix Long (96% over 26bp)
CAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG16250.11289410463511931Clontech Universal Primer Mix Long (96% over 25bp)
TCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT16060.11157411202707791Clontech SMART CDS Primer II A (96% over 25bp)
TGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTA15990.11108779896095738Clontech SMART CDS Primer II A (96% over 26bp)
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC15830.10997622623839622Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGT15780.10962885976259587Clontech SMART CDS Primer II A (96% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15590.10830886715455447Clontech SMART CDS Primer II A (100% over 25bp)
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA15300.10629414159491234Clontech Universal Primer Mix Long (96% over 25bp)
AGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCAC15100.10490467569171087Clontech SMART CDS Primer II A (100% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC15040.10448783592075042Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC14910.10358468308366948Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA14890.10344573649334933Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT14410.10011101832566581Clontech Universal Primer Mix Long (100% over 23bp)

[WARN]Adapter Content

Adapter graph