FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001601893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001601893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1393706
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATTAAGGGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGA21270.15261468344112747No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA19470.13969947750816886Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC18700.13417463941462546Clontech Universal Primer Mix Long (96% over 25bp)
TAACAATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGA18700.13417463941462546No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)