FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001601894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001601894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1393706
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC20730.1487401216612399Clontech Universal Primer Mix Long (96% over 25bp)
TTATTAAGGGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGA20130.14443505301692036No Hit
TAACAATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGA19420.1393407217878089No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA17880.1282910456007221Clontech SMART CDS Primer II A (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)