FastQCFastQC Report
Fri 9 Sep 2022
EGAF00001601902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001601902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1635547
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT202841.2401967048333065Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG189851.160773735025652Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA99710.6096431346821582Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG83820.5124890938627872Illumina Single End PCR Primer 1 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC60800.3717410750042646Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG40240.246033895693612Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACG38270.2339889957304804Illumina Single End PCR Primer 1 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA27210.16636635938924407Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTC24810.15169236958644416Illumina Single End PCR Primer 1 (97% over 34bp)
ATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATA18510.1131731463540944No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG16970.1037573362306311No Hit
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCT16960.10369619460645275Illumina Single End PCR Primer 1 (97% over 34bp)
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCC16530.10106710476678443No Hit
ATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGT16370.10008883877993112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)