FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001601905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001601905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56546
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC988617.48311109539136Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC848415.003713790542214Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT37966.713118522972447TruSeq Adapter, Index 1 (97% over 35bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGA34406.083542602482934TruSeq Adapter, Index 7 (97% over 37bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAG28585.054292080783786TruSeq Adapter, Index 19 (97% over 38bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG26144.622784989212322Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC13872.4528702295476252Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCT9841.740176139780002TruSeq Adapter, Index 19 (97% over 38bp)
ATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG9601.6977328192975631TruSeq Adapter, Index 7 (97% over 36bp)
TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG9221.6305308952003679TruSeq Adapter, Index 7 (97% over 36bp)
TATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAA9141.6163831217062214TruSeq Adapter, Index 19 (97% over 38bp)
ATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAA8751.547412725922258TruSeq Adapter, Index 19 (97% over 38bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT6181.092915502422806Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC4120.7286103349485376Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA3620.6401867506101228Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3380.5977434301276836Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC3340.5906695433806105Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGC2480.4385809783185371TruSeq Adapter, Index 19 (97% over 38bp)
ATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGC2270.4014430728964029TruSeq Adapter, Index 19 (97% over 38bp)
TATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1660.29356630000353695Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG1660.29356630000353695Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1440.25465992289463446Clontech SMART CDS Primer II A (100% over 26bp)
ATATTTATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1220.21575354578573197Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGT1140.2016057722915856Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATATTTATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC800.14147773494146357Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC800.14147773494146357No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGAC670.11848760301347576Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)