FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001601906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001601906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56546
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA981617.35931807731758Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG829214.6641672266827Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGG37156.569872316344215Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCT34386.080005659109398Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT28475.0348388922293354Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG25064.431790047041346Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG12392.1911364199059173Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAG9741.722491422912319Illumina Single End PCR Primer 1 (97% over 35bp)
TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGC9271.6393732536342094Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGC9251.6358363102606726Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTA9231.6322993668871362Illumina Single End PCR Primer 1 (97% over 35bp)
TATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTA8781.552718140982563Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA5410.9567431825416475Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG4000.7073886747073179Illumina Single End PCR Primer 1 (100% over 35bp)
TATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT3370.5959749584409153Illumina Single End PCR Primer 1 (100% over 33bp)
ATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG3310.5853641283203056Illumina Single End PCR Primer 1 (100% over 35bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG3110.5499946945849397Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATA2500.44211792169207376Illumina Single End PCR Primer 1 (97% over 35bp)
TATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATA2240.39613765783609806Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT1660.29356630000353695Illumina Single End PCR Primer 1 (100% over 33bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG1510.2670392247020125Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT1200.2122166024121954Illumina Single End PCR Primer 1 (100% over 31bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGAG690.12202454638701235Illumina Single End PCR Primer 1 (96% over 32bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT680.12025607470024405No Hit
ATATATATATATATATATAGATCGGAAGAGCGTTGTGTAGGGAAAGAGTG640.11318218795317087Illumina Single End PCR Primer 1 (96% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCGGCGTGTAGGGAAAGAGTG600.1061083012060977Illumina Single End PCR Primer 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)