FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001601911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001601911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52781
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC900517.061063640325116Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC769414.577215285803605Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT36766.964627422746822TruSeq Adapter, Index 1 (97% over 35bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGA33106.271196074344934TruSeq Adapter, Index 7 (97% over 37bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAG31515.969951308235919TruSeq Adapter, Index 19 (97% over 38bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG23644.478884447054812Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC13052.472480627498532Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCT12692.404274265360641TruSeq Adapter, Index 19 (97% over 38bp)
TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG9451.7904170061196263TruSeq Adapter, Index 7 (97% over 36bp)
ATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG9411.7828385214376388TruSeq Adapter, Index 7 (97% over 36bp)
TATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAA9271.7563138250506811TruSeq Adapter, Index 19 (97% over 38bp)
ATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAA8461.6028495102404272TruSeq Adapter, Index 19 (97% over 38bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT5701.0799340671832667Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC4080.7730054375627593Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA3410.646065819139463Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC3150.5968056687065422Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGC3020.5721755934900816TruSeq Adapter, Index 19 (97% over 38bp)
ATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2970.5627024876375969Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGC2850.5399670335916333TruSeq Adapter, Index 19 (97% over 38bp)
TATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1480.28040393323354995Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG1390.2633523426990773Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1180.2235652981186412Clontech SMART CDS Primer II A (100% over 26bp)
ATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGT1160.21977605577764725Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC1060.20082984407267768No Hit
ATATTTATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1050.1989352229021807Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG820.15535893598075065Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATTTATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC800.15156969363975673Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG620.11746651257081146Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG600.11367727022981754Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA590.1117826490593206Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGAC530.10041492203633884Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)