FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001601912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001601912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52781
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG786914.908773990640572Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG684312.964892669710693Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCC32376.132888728898657Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTC28395.378829503040867Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTA26415.003694511282469Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG21254.026069987306038Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG11132.108713362763116Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATG10682.0234554100907522Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCT8441.5990602678994337Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCT7901.4967507246925977Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCT7831.4834883764991191Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCT6881.3034993653019078Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA4590.8696311172581043Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGC3540.6706958943559235Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGAG3160.598700289877039Illumina Single End PCR Primer 1 (96% over 32bp)
ATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGC2870.5437562759326273Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTAT2560.48502301964722155Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTG2550.48312839847672456Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG2510.47554991379473677Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT2490.47176067145374284Illumina Single End PCR Primer 1 (100% over 33bp)
TATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTAT2410.4566037020897672Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGCG1570.2974555237680226Illumina Single End PCR Primer 1 (96% over 32bp)
ATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT1390.2633523426990773Illumina Single End PCR Primer 1 (100% over 33bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTG1330.2519846156760956Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG1100.2084083287546655Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT1050.1989352229021807Illumina Single End PCR Primer 1 (100% over 31bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGCGTG960.18188363236770808Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTGCC940.17809439002671415Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCGCTA890.16862128417422936Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG830.1572535571512476Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG820.15535893598075065Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGAG810.1534643148102537Illumina Single End PCR Primer 1 (96% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGCG800.15156969363975673Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT760.1439912089577689Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGG740.14020196661677498Illumina Single End PCR Primer 1 (96% over 32bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTGCCTC710.13451810310528411Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA700.13262348193478712Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGG660.1250449972527993Illumina Single End PCR Primer 1 (96% over 32bp)
ATATATATATATATATATATAGATCGGAAGAGCGGCGTGTAGGGAAAGAG640.12125575491180539Illumina Single End PCR Primer 1 (96% over 30bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG620.11746651257081146Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT620.11746651257081146No Hit
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTGCCTCTA570.10799340671832666Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGCGCC570.10799340671832666Illumina Single End PCR Primer 1 (96% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCGTTGTGTAGGGAAAGAGTG560.10609878554782971Illumina Single End PCR Primer 1 (96% over 32bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCGC540.1023095432068358Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGGCGTGTAGGGAAAGAGTG540.1023095432068358Illumina Single End PCR Primer 1 (96% over 32bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCCC530.10041492203633884Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCAC530.10041492203633884Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)