FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001601913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001601913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069941
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT150091.4027876303459723Clontech SMART CDS Primer II A (100% over 26bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC126961.1866074858333309Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC124571.164269805531333Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG76220.7123757291289894Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA66110.6178845375586131Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT51400.480400321139203Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG51310.4795591532617219Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG46330.43301453070776796TruSeq Adapter, Index 2 (100% over 35bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC38310.3580571265144527Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCT32250.30141848943072563TruSeq Adapter, Index 2 (97% over 37bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA28060.2622574515791058Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG28050.2621639884816079Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG27880.2605751158241436Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCA20100.18786082597077783TruSeq Adapter, Index 2 (97% over 37bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA19420.18150533534092067Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT18850.1761779387835404Clontech SMART CDS Primer II A (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG18820.17589754949104672Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC18640.1742152137360845Clontech Universal Primer Mix Long (96% over 25bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC18200.17010283744617694Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA16580.15496181565151723Clontech SMART CDS Primer II A (96% over 26bp)
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG15730.14701745236419578No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA15110.141222740319326Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC14480.13533456517695835Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCT14230.13299798773951085Clontech SMART CDS Primer II A (96% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC14210.13281106154451508Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA13050.12196934223475875Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATA13000.12150202674726925No Hit
GTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAG12980.12131510055227344No Hit
ATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCT12800.11963276479731125No Hit
CTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTC12750.11916544930982176Clontech SMART CDS Primer II A (96% over 26bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG12700.11869813382233226Clontech Universal Primer Mix Long (96% over 25bp)
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12500.11682887187237428No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC12300.11495960992241629Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCC11760.10991260265752971No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT11680.10916489787754652Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCG11510.10757602522008224No Hit
GTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGT11270.10533291088013265No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC11240.10505252158763895Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGT11180.10449174300265154No Hit
ATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATG10930.10215516556520406No Hit
ATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAA10910.10196823937020827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)