FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001601915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001601915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52732
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC869816.49472805886369Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC717213.600849579003263Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAG37787.16453007661382TruSeq Adapter, Index 19 (97% over 38bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT36726.963513616020632TruSeq Adapter, Index 1 (97% over 35bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGA34676.574755366760221TruSeq Adapter, Index 7 (97% over 37bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG20863.955852233937647Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCT20573.9008571645300765TruSeq Adapter, Index 19 (97% over 38bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC10732.0348175680801033Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAA10101.9153455207464156TruSeq Adapter, Index 19 (97% over 38bp)
ATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG9671.833801107486915TruSeq Adapter, Index 7 (97% over 36bp)
ATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAA9431.7882879465978911TruSeq Adapter, Index 19 (97% over 38bp)
TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG9391.7807024197830539TruSeq Adapter, Index 7 (97% over 36bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT5190.9842221042251384Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGC4750.9007813092619282TruSeq Adapter, Index 19 (97% over 38bp)
ATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGC4250.8059622240764621TruSeq Adapter, Index 19 (97% over 38bp)
ATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC4120.7813092619282409Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA3670.6959720852613215Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC3140.5954638549647273Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2710.5139194417052264Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG1710.3242812713342942Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1670.31669574451945687Clontech SMART CDS Primer II A (100% over 26bp)
TATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1570.29773192748236366Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATATTTATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1070.20291284229689752Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1030.19532731548206023Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC960.182052643556095No Hit
ATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGT860.16308882651900172Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA690.13085033755594327Clontech Universal Primer Mix Long (96% over 26bp)
ATATTTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAG690.13085033755594327TruSeq Adapter, Index 19 (97% over 38bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG680.12895395585223393Clontech SMART CDS Primer II A (96% over 26bp)
ATATTTATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC670.12705757414852462Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA630.11947204733368733Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG610.11567928392626867Clontech Universal Primer Mix Long (96% over 26bp)
ATATTTATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT590.11188652051885005TruSeq Adapter, Index 1 (97% over 35bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGAC560.10619737540772207Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA560.10619737540772207Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATTTATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGA540.10240461200030343TruSeq Adapter, Index 7 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)