FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001601916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001601916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52732
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA862816.361981339604036Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG696213.202609421224304Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG38377.276416597132671Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGG36566.9331715087612835Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAT35156.665781688538269Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGG20843.9520594705302283Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG20293.8477584768262156Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATA10361.9646514450428583Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG9751.8489721611165897Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGA9531.8072517636349845Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGA9521.8053553819312749Illumina Single End PCR Primer 1 (97% over 35bp)
TATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATA9251.7541530759311235Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGG4870.9235378897064401Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA4440.8419934764469392Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGG4230.8021694606690435Illumina Single End PCR Primer 1 (97% over 35bp)
TATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG4040.7661382082985663Illumina Single End PCR Primer 1 (100% over 35bp)
ATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG3210.6087385268906925Illumina Single End PCR Primer 1 (100% over 35bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG3000.5689145111127968Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT2670.5063339148903891Illumina Single End PCR Primer 1 (100% over 33bp)
ATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT1630.3091102177046196Illumina Single End PCR Primer 1 (100% over 33bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1360.25790791170446786Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG1320.2503223848896306Illumina Single End PCR Primer 1 (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA1100.2086019874080255Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT840.15929606311158312Illumina Single End PCR Primer 1 (100% over 31bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG690.13085033755594327Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA680.12895395585223393Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT680.12895395585223393No Hit
ATATATATATATATATATAGATCGGAAGAGCGTTGTGTAGGGAAAGAGTG620.117575665629978Illumina Single End PCR Primer 1 (96% over 32bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA600.11378290222255935Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAGAGAGTG530.1005082302965941Illumina Single End PCR Primer 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)