FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001601918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001601918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81311
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT74629.177110108103454Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG61487.5610925950978345Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCA32333.976091795697999Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGA31083.8223610581594123Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGC28223.470625130671127Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG17592.163298938642988Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCG10721.3183948051309171Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAG9261.1388373036858481Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAG9001.1068613102778222Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG8961.1019419266765873Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAG7990.9826468743466444Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAG7870.9678887235429401Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5200.6395198681605194Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA4370.5374426584348981Illumina Single End PCR Primer 1 (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA4140.5091562027277982Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTC3590.4415146782108202Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTC2740.33697777668458145Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCC2740.33697777668458145Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG2660.3271390094821119Illumina Single End PCR Primer 1 (100% over 24bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA2650.32590916358180316Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG2620.32221962588087716Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA2600.3197599340802597Clontech Universal Primer Mix Long (96% over 26bp)
TATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCC2580.3173002422796424Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT2350.28901378657254245Illumina Single End PCR Primer 1 (100% over 33bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG2340.2877839406722338Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA1650.20292457355093407Illumina Single End PCR Primer 1 (100% over 34bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1410.17340827194352548Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT1390.1709485801429081Illumina Single End PCR Primer 1 (100% over 33bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA1340.16479935064136464Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1330.16356950474105594Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG1300.15987996704012986Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC1270.1561904293392038Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA1230.15127104573796904Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1170.1438919703361169Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG1160.14266212443580817Illumina Single End PCR Primer 1 (100% over 22bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT1130.1389725867348821Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC1110.13651289493426474Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC1100.13528304903395605Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC980.12052489823025174Clontech Universal Primer Mix Long (96% over 25bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGAG900.11068613102778221Illumina Single End PCR Primer 1 (96% over 32bp)
CTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCT880.10822643922716484Clontech SMART CDS Primer II A (96% over 25bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT880.10822643922716484Clontech SMART CDS Primer II A (96% over 26bp)
CTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTC850.10453690152623876Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC840.10330705562593007Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGCT830.10207720972562138Illumina Single End PCR Primer 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)