FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001601920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001601920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100778
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC1447614.364246164837564Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1222912.13459286749092Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCG58115.766139435194189Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTT56665.6222588263311435Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCC48064.768897973764115Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG36333.604953462065133Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG18731.8585405544860982Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTG17551.741451507273413Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGC16231.6104705392049852Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTC16211.6084859790827362Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTC14451.4338446883248328Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGC14381.4268987278969616Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA9100.9029748556232511Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCT5610.5566691142908174Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCT5100.5060628311734704Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG5010.49713231062335034Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCT4650.46141022842287005Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT4370.4336263867113855Illumina Single End PCR Primer 1 (100% over 33bp)
TATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCT4300.42668042628351427Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGGAGGGAAAGAGTG3310.3284447002321935Illumina Single End PCR Primer 1 (96% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTC3140.31157593919307786Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGAG3020.29966857845958444Illumina Single End PCR Primer 1 (96% over 32bp)
ATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT2700.26791561650360196Illumina Single End PCR Primer 1 (100% over 33bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTC2520.25005457540336185Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG2410.23913949473099286Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGGAGGGAAAGAG2060.20440969259163705Illumina Single End PCR Primer 1 (96% over 30bp)
TATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT1730.17166445057453017Illumina Single End PCR Primer 1 (100% over 31bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGGAGGGAAAG1590.15777252971878783Illumina Single End PCR Primer 1 (96% over 28bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTG1530.15181884935204112Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT1460.14487288892416997No Hit
ATATATATATATATAGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTCTT1260.12502728770168092Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTCTT1010.10022028617356962Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)