FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001601944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001601944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743962
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA133431.7935055822743635Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA121161.628577803705028Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC115611.5539772192665755Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT109961.4780324801535563Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA61370.8249077237815909Clontech Universal Primer Mix Long (96% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT51350.6902234253900065Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG47870.6434468427150849Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG44500.5981488301821867Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG44220.5943851970933999Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG43960.5908903949395264Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC35530.47757815587355273Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC34490.46359894725805884Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC34050.4576846666899653Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC32980.44330221167210154Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG32640.4387320857785747Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT29850.40123017035816344Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA28010.376497724346136Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA27100.3642659168075789Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC24500.32931789526884436Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG20600.27689586296074264Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG19020.25565821910258857Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG18770.25229783241617176Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA16360.21990370475911403Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA16260.21855955008454733Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA15560.20915046736258036Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC15410.20713423535073028Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC14780.19866606090096Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT14520.19517125874708655Clontech Universal Primer Mix Long (100% over 23bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC13880.1865686688298596Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA13850.18616542242748957Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT13610.18293945120852947Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC13510.18159529653396275Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG12070.1622394692202021Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC11760.15807258972904528Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC11530.15498103397754187Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11220.15081415448638505Clontech Universal Primer Mix Long (96% over 26bp)
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11110.14933558434436167No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10560.14194273363424476Clontech Universal Primer Mix Long (96% over 26bp)
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG10420.14006091708985136No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10140.13629728400106458Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10030.1348187138590412Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC9520.12796352501875094Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA9440.12688820127909758Clontech Universal Primer Mix Long (96% over 25bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG9420.12661937034418425Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG9190.12352781459268079Clontech SMART CDS Primer II A (96% over 26bp)
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG9020.12124275164591738No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA8930.12003301243880735Clontech Universal Primer Mix Long (96% over 26bp)
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC8640.11613496388256389Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA8630.11600054841510724Clontech Universal Primer Mix Long (96% over 25bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG8590.11546288654528053Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG8550.11492522467545385Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8350.11223691532632042Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG8280.11129600705412374Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA8260.11102717611921038Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG8090.10874211317244697Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7650.10282783260435345No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)