FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001602103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852971
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC212812.494926556705914Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC176442.0685345691705814Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT90821.0647489773978247Clontech SMART CDS Primer II A (100% over 26bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA72350.8482117211487847TruSeq Adapter, Index 7 (97% over 35bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG57780.6773970041185456Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT48240.5655526389525553Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA46560.5458567759044564TruSeq Adapter, Index 7 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG43620.5113890155702832Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG40840.47879705171688136Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATT29880.3503049927840454TruSeq Adapter, Index 7 (97% over 38bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA28510.3342434854174409Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC20440.23963300041853708Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC19650.23037125529472868Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA18460.2164200189689919Clontech Universal Primer Mix Long (96% over 26bp)
TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG14020.1643666666275876TruSeq Adapter, Index 7 (97% over 36bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG13830.16213915830667164Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC13450.15768414166483974Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG13240.15522215878382736TruSeq Adapter, Index 7 (97% over 36bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG11590.13587800757587304Clontech SMART CDS Primer II A (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC11090.13001614357346264Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10580.12403704229100403Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG10300.12075439844965422No Hit
TATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT10060.11794070372849721TruSeq Adapter, Index 7 (97% over 38bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT9930.11641661908787052Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC9600.11254778884627965Clontech Universal Primer Mix Long (96% over 25bp)
ATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT9380.10996856868521908TruSeq Adapter, Index 7 (97% over 38bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA9330.10938238228497804Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAG9050.10609973844362822No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC8790.10305156916237482Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG8720.10223090820203734Clontech Universal Primer Mix Long (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)