FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001602107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888757
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT677987.628406864868575Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG469185.279058280272335Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA238692.6856609849486417Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT89571.0078120341105612Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG75580.8504011782748266Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT52700.5929629808822884Clontech SMART CDS Primer II A (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT50700.5704596419493742Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT48190.5422179515885669Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC46310.5210648129916277Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC43770.4924855725468267Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC43450.48888503831756036Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG43080.48472192061497127Clontech Universal Primer Mix Long (96% over 26bp)
TAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT42660.4799962194390593Clontech SMART CDS Primer II A (100% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA39330.44252816011575724Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG37720.42441297227476127Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA36630.41214865255632305Clontech Universal Primer Mix Long (96% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35970.40472255070846136Clontech SMART CDS Primer II A (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35660.40123453317385965Clontech SMART CDS Primer II A (100% over 24bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC34260.3854821959208198Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA33300.37468059323302094Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT33290.3745680765383564Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT32570.3664668745225073Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG31710.3567904387813542Clontech Universal Primer Mix Long (96% over 26bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC29880.3361998836577377Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC27590.31043356057955096Clontech SMART CDS Primer II A (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA26740.3008696415330625Clontech SMART CDS Primer II A (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG26500.2981692408611128Clontech SMART CDS Primer II A (96% over 26bp)
CTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTC26130.29400612315852365Clontech SMART CDS Primer II A (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC25930.2917557892652322Clontech Universal Primer Mix Long (96% over 25bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT25390.2856798877533454Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23660.26621449957637466Clontech SMART CDS Primer II A (100% over 23bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC23370.26295151543110207Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA22790.256425547140557Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC21960.24708666148339758Clontech SMART CDS Primer II A (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21220.23876042607821935No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC20950.23572247532227594Clontech Universal Primer Mix Long (96% over 25bp)
TGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC20580.23155935761968682Clontech SMART CDS Primer II A (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC19730.22199543857319828Clontech Universal Primer Mix Long (96% over 26bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG19610.22064523823722343Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG18450.20759330165613324Clontech SMART CDS Primer II A (96% over 26bp)
ACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCT17870.20106733336558813Clontech SMART CDS Primer II A (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT17350.19521646524303043Clontech Universal Primer Mix Long (96% over 25bp)
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG17220.193753748212391Clontech Universal Primer Mix Long (96% over 26bp)
TGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA17080.192178514487087Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC16630.18711526322718133Clontech SMART CDS Primer II A (96% over 26bp)
TGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTA16480.18542751280721276Clontech SMART CDS Primer II A (96% over 26bp)
CACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC16470.1853149961125482Clontech SMART CDS Primer II A (96% over 26bp)
TTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT16300.1834022123032505Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT16010.18013922815797795Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT15490.17428836003542025Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC14690.16528702446225457Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT14090.15853602278238033Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG13900.1563982055837535Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT13580.1527976713544872Clontech SMART CDS Primer II A (96% over 25bp)
TAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA12660.1424461354453467Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT12320.13862056782675128Clontech Universal Primer Mix Long (96% over 26bp)
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA11670.13130698267355417Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCT11540.12984426564291476Clontech SMART CDS Primer II A (96% over 25bp)
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA11520.12961923225358563Clontech SMART CDS Primer II A (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC11330.12748141505495877Clontech Universal Primer Mix Long (96% over 26bp)
TACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT11290.1270313482763005Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG10690.12028034659642625Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10530.11848007948179311Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA10480.11791749600847026Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCC10150.11420444508453943Clontech SMART CDS Primer II A (96% over 26bp)
ACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCG10060.11319179483255828Clontech SMART CDS Primer II A (96% over 26bp)
CTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA9420.10599072637402573Clontech SMART CDS Primer II A (96% over 26bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA9280.10441549264872176Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9280.10441549264872176Clontech SMART CDS Primer II A (100% over 22bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA9010.10137754189277835Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)