FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001602119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717745
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT424421.5616623340306026Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG250080.92017463007015Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG206580.7601154633712877Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC196700.7237617951647414Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA191860.7059529131688219Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT167900.6177915882468737Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC162910.5994307780899238Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC122320.4500790177150542Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT73560.27066557016938675TruSeq Adapter, Index 1 (100% over 35bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA68230.25105372284743416Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG56930.2094751347164653Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTC54580.20082826019365319TruSeq Adapter, Index 13 (97% over 37bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG54450.2003499224540934Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC52950.1948306408437878Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA46860.17242235750594703Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT43120.15866094869091837Clontech SMART CDS Primer II A (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC41160.15144908738678572Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT39420.14504672071883123Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG39320.14467876861147755Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC38630.14213989907073696Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA36640.1348176521343982Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAG36560.13452329044851521TruSeq Adapter, Index 13 (97% over 38bp)
GTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGT33500.12326395596349178No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG33490.12322716075275642Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC33380.12282241343466735Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG32940.12120342416231103No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC32230.11859096420009972Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA31810.11704556534921415Clontech SMART CDS Primer II A (96% over 26bp)
CTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTC31530.11601529944862378Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC31410.11557375691979932Clontech Universal Primer Mix Long (96% over 25bp)
ATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGG30860.11355002032935393No Hit
GTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAG30580.11251975442876355No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG30480.11215180232140984No Hit
CTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCT29230.10755240097948852Clontech SMART CDS Primer II A (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC29200.1074420153472824Clontech Universal Primer Mix Long (96% over 25bp)
ATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGT28950.10652213507889813No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA28500.10486635059580646No Hit
ATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATA28420.10457198890992349No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCC28090.10335774695565626No Hit
ATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCT27810.10232748105506587No Hit
ATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGA27600.10155478162962309No Hit
ATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCG27200.10008297320020826No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)