FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001602120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717745
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG334641.2313149320484444Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG298591.0986681973474333Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA251820.9265769967381046Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT250550.9219040049747125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA180270.6633072639265273Illumina Single End PCR Primer 1 (100% over 34bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC174390.6416716800141293Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG150410.5534367646707105Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACG67310.24766856345978006Illumina Single End PCR Primer 1 (97% over 34bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA59520.21900509429692633Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG53590.1971855343308515Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG49080.1805908942891993Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTC45820.16859565558946848Illumina Single End PCR Primer 1 (97% over 34bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA44920.16528408662328511Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC43930.1616413607604834Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT36680.13496483297733966Clontech SMART CDS Primer II A (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC35620.13106454063939038Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG35390.13021825079247684Illumina Single End PCR Primer 1 (100% over 30bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT35020.12885682799526815Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA34280.1261339824008507Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGT31510.11594170902715303No Hit
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCT31390.11550016649832857Illumina Single End PCR Primer 1 (97% over 34bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC31210.1148378527050919Clontech Universal Primer Mix Long (96% over 25bp)
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG30610.11263014006096966No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC29590.10887702856596185Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGT29390.10814112435125443No Hit
GTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAG29350.10799394350831296No Hit
CTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTC28260.10398326553815755Clontech SMART CDS Primer II A (96% over 26bp)
ATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGG27880.10258504753021347No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG27830.10240107147653661Clontech Universal Primer Mix Long (96% over 26bp)
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG27820.10236427626580125No Hit
ATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATA27760.10214350500138902No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC27350.10063490136123883Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC27330.10056131093976808Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA27320.10052451572903272Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)