FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001602191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1243999
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT778536.258284773540815Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG333172.678217586991629Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA238421.9165610261744583Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT150701.2114157648036694Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG150491.2097276605527818Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC56670.4555469899895418Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT54190.4356112826457256Clontech Universal Primer Mix Long (96% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37120.29839252282357137Clontech SMART CDS Primer II A (100% over 25bp)
TAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT33460.2689712773081007Clontech SMART CDS Primer II A (100% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT28620.23006449362097556Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT27000.21704197511412793Clontech SMART CDS Primer II A (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26640.2141480821126062Clontech SMART CDS Primer II A (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG25140.2020901946062658Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC25050.20136672135588532Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA24630.19799051285411Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC24590.19766896918727425Clontech SMART CDS Primer II A (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC24320.19549854943613298Clontech SMART CDS Primer II A (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA24120.19389083110195426Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG23750.1909165521837236Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG21940.17636670125940618Clontech SMART CDS Primer II A (96% over 26bp)
CTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTC21540.1731512645910487Clontech SMART CDS Primer II A (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA20980.16864965325534825Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20540.16511267292015508Clontech SMART CDS Primer II A (100% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19530.1569936953325525No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC19040.15305478541381465Clontech Universal Primer Mix Long (96% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC18940.1522509262467253Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG18930.15217054033001634Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA18810.15120590932950911Clontech SMART CDS Primer II A (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC18550.14911587549507677Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT17810.14316731765861548Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA16660.1339229372370878Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT16180.13006441323505888Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT15700.12620588923302992Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG15130.12162389198062057Clontech Universal Primer Mix Long (96% over 26bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT15070.12114157648036694Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCT14700.11816729756213631Clontech SMART CDS Primer II A (96% over 26bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC14690.11808691164542737Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC14640.11768498206188267Clontech Universal Primer Mix Long (96% over 26bp)
CACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC14590.117283052478338Clontech SMART CDS Primer II A (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC14180.11398722989327162Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG13230.10635056780592268Clontech SMART CDS Primer II A (96% over 26bp)
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC12620.10144702688667756Clontech SMART CDS Primer II A (96% over 26bp)
TGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTA12500.10048239588617033Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)