FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001602196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681480
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG183382.690908023713095Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA123851.817368081234959Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT100761.47854669249281Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG76421.121382872571462Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35960.5276750601631743Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC18390.26985384750836416Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG17070.25048424018313087Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12600.184891706286318Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG10920.16023947878147562Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10090.148060104478488Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT9060.13294594118682868Clontech SMART CDS Primer II A (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG9030.1325057228385279Clontech SMART CDS Primer II A (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC8710.12781006045665316Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC8300.12179374302987615Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC8130.1192991723895052Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA8040.11797851734460293Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC7880.11563068615366556Clontech SMART CDS Primer II A (96% over 26bp)
CTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTC7710.1131361155132946Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA7630.11196219991782591Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG7300.10711979808651759Clontech Universal Primer Mix Long (96% over 26bp)
TAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA7210.1057991430416153Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTCCGGAATTCATGTATACAG7110.10433174854727946Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA7070.10374479074954511Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)