FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001602216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680078
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG153032.2501830672364056Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA82101.2072144665758926Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG59540.8754878116921883Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG58490.8600484062122287Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTC56510.8309340987357333Illumina Single End PCR Primer 1 (96% over 33bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG46860.6890386102770565Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA40000.5881678278079868Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT36820.5414084854972517Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTA36820.5414084854972517Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCC36010.52949808698414Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG35880.5275865415437642Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC33710.4956784368851808Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC29090.4277450527733584Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA26960.39642511594258306Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA24710.3633406756283838Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG23050.33893171077435236Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC22990.3380494590326404Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC21400.3146697878772729Clontech Universal Primer Mix Long (96% over 25bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT19680.2893785712815295Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG18400.2705572007916739Illumina Single End PCR Primer 1 (100% over 30bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG18370.2701160749208179Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT17580.25849976032161015Clontech SMART CDS Primer II A (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA16550.24335443875555451Clontech SMART CDS Primer II A (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA14690.21600463476248313Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC14200.20879957887183528Clontech Universal Primer Mix Long (96% over 25bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG14150.20806436908707532Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC13900.20438832016327538Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC13820.2032119845076594Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA12980.1908604601236917Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCT12130.17836189378277198Clontech SMART CDS Primer II A (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC11970.17600922247154005Clontech Universal Primer Mix Long (96% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11720.1723331735477401Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT10950.16101094286243636Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG10900.1602757330776764Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG10680.15704081002473244Illumina Single End PCR Primer 1 (100% over 28bp)
CTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTC10500.1543940547995965Clontech SMART CDS Primer II A (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10400.15292363523007657Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCT9920.14586562129638073Illumina Single End PCR Primer 1 (96% over 33bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG9720.14292478215734078Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC9660.14204253041562878Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCT9490.13954281714744485Illumina Single End PCR Primer 1 (96% over 33bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA9160.13469043256802898Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG8990.13219071929984502Clontech Universal Primer Mix Long (96% over 26bp)
TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCT8840.12998508994556507Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCT8430.1239563697105332Illumina Single End PCR Primer 1 (96% over 33bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG8310.12219186622710923Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA8260.12145665644234926Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA7880.1158690620781734Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG7610.11189892924046947Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7540.1108696355418055Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA7420.10910513205838153Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG7410.10895809010142954Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT7390.10866400618752554Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT7310.10748767053190957Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG7060.10381162160810965Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCG7000.10292936986639768Clontech SMART CDS Primer II A (96% over 26bp)
ATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCG6830.10042965659821372No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)