FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001602226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519845
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA81011.5583491232963673Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG76531.4721695890121094Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAT74291.4290798218699805Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG47340.9106560609412421Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGG47120.906424030239783Illumina Single End PCR Primer 1 (97% over 35bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG32320.6217237830507171Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT25780.49591705219825144Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG22460.43205186161259607Illumina Single End PCR Primer 1 (100% over 30bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC20570.39569487058642483Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATA12840.24699670093970316Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGA12310.23680135424982446Illumina Single End PCR Primer 1 (97% over 35bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA12120.2331464186440189Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG11720.22545181736863873Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGA11570.22256634189037117Illumina Single End PCR Primer 1 (97% over 35bp)
TATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATA10230.19678942761784762Illumina Single End PCR Primer 1 (97% over 35bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG9580.18428570054535487Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG6340.12195943021477557Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA5840.11234117862055036Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA5630.10830151295097576Illumina Single End PCR Primer 1 (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5580.10733968779155324Clontech SMART CDS Primer II A (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)