FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001602230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933189
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA330043.536689780955412Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG270782.9016630071721807Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT178201.9095810173501833Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG138911.4885516224473285Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGA118491.2697320692807137Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAA89730.9615415526758245Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATT87170.9341087389585603Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG49550.5309749686290773Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG45710.4898257480531811Clontech SMART CDS Primer II A (96% over 26bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA44840.48050287776645456Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT44170.4733231960513894Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT38070.40795594461572093Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG37880.4059199154726427Illumina Single End PCR Primer 1 (100% over 30bp)
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA31180.3341230983219905Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGAT30700.3289794457500035Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA30400.3257646628925116Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG28780.3084048354620554Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTC28210.3022967480328208Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAG27550.29522422574633866Illumina Single End PCR Primer 1 (96% over 33bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC27320.2927595588889282Clontech Universal Primer Mix Long (96% over 26bp)
TATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGAT27250.2920094428888467Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG27060.28997341374576857Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC27050.2898662543171855Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCT26460.28354384803078475Clontech SMART CDS Primer II A (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG24880.2666126583146608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA24870.2665054988860778Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC24840.26618402060032853Clontech Universal Primer Mix Long (96% over 25bp)
TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAG24460.26211196231417216Illumina Single End PCR Primer 1 (96% over 33bp)
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG23610.2530034108846118Clontech SMART CDS Primer II A (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC22260.2385368880258983Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT21800.23360755431107738Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC21370.2289996988820057Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG20710.22192717659552352Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC19220.2059604217366471Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG19070.2043530303079012Illumina Single End PCR Primer 1 (100% over 28bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC17910.19192253659226588Clontech Universal Primer Mix Long (96% over 26bp)
ACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCG17430.18677888402027884Clontech SMART CDS Primer II A (96% over 26bp)
CTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA17200.1843142171628684Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17180.18409989830570225Clontech SMART CDS Primer II A (100% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC17010.1822781880197902Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG16970.18184955030545796Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA16760.17959920230521365Clontech SMART CDS Primer II A (96% over 26bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG16710.17906340516229832Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC16480.17659873830488784Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC15590.16706154916099525Clontech SMART CDS Primer II A (96% over 26bp)
TTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGAT15370.16470404173216788Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA15220.16309665030342194Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT14720.1577386788742688Clontech Universal Primer Mix Long (96% over 25bp)
ACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCT14490.1552740120168583Clontech SMART CDS Primer II A (96% over 26bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG13720.14702273601596247Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA13130.14070032972956176Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC12030.12891279258542482Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA11760.12601948801368212Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11100.11894696572719995Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11080.11873264687003383Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10890.11669661772695564Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10640.11401763201237906Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA10380.11123148686921942Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAG10120.10844534172605978Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGTACATGTAAGCAGTGGTATCAACGCAGA9960.10673079086873077Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT9700.10394464572557112Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC9620.10308737029690662Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG9610.10298021086832355Clontech SMART CDS Primer II A (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA9590.10276589201115745Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT9370.10040838458233005Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)