FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001602383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182100
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT148841.259115134083411Clontech SMART CDS Primer II A (100% over 26bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC124971.05718636325184Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC123421.0440741054056342Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG44870.37957871584468317TruSeq Adapter, Index 2 (100% over 35bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG41080.34751713053041194Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCT33270.2814482700279164TruSeq Adapter, Index 2 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA29220.24718720920395906Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG27010.22849166737162677Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG23600.19964470010997376Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCA19050.16115387869046613TruSeq Adapter, Index 2 (97% over 37bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG18130.15337111919465357Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA18120.15328652398274256Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC16460.13924371880551561Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC15190.12850012689281787Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA13810.11682598764909904Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG13120.11098891802723966Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC12340.1043904914981812Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)