FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001602397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2176865
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT259391.1915759590052668Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG228371.0490774577201618Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT179760.8257746805612658Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC85400.39230728593642694Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC80000.36750097043225005Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG59150.27172103001334486Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC55840.2565156773617105Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA41260.18953862550043296Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA31870.14640319909594762TruSeq Adapter, Index 7 (97% over 35bp)
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG27620.12687971004173434No Hit
ATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATA26660.12246969839654732No Hit
ATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCT24580.11291467316530883No Hit
ATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGA24400.11208779598183627No Hit
GTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAG23960.1100665406444589No Hit
ATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGA23800.1093315387035944No Hit
GTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAA23320.10712653288100088No Hit
GTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAG22700.10427840036015094No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTC22600.10381902414711064TruSeq Adapter, Index 7 (97% over 35bp)
GTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGT22410.10294620934233406No Hit
ATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGT22040.1012465173540849No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)