FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001602399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65133
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC994715.271828412632612Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC981415.067630847650193Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA40186.168915910521548TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAT39306.033807747224909TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTC35465.444244852839574TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG33165.091121244223358Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC19522.996944713125451Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT10471.607480079222514Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGT9571.4693012758509512TruSeq Adapter, Index 7 (97% over 35bp)
TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAA8581.317304592142232TruSeq Adapter, Index 7 (97% over 35bp)
TATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATT8521.3080926719174613TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAA8191.2574271106812216TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATT7541.1576313082462037TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA6510.9994933443876376Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG4150.6371578155466507Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG3530.5419679732240186Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG2910.44677813090138635Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2580.3961125696651467Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2410.37001212902829594Clontech SMART CDS Primer II A (100% over 26bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC2270.34851764850383066No Hit
TATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCG1720.2640750464434311TruSeq Adapter, Index 7 (97% over 35bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG1640.25179248614373667Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA1430.21955076535703869Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1400.21494480524465323Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCG1250.19191500468272613TruSeq Adapter, Index 7 (97% over 35bp)
TATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1220.1873090445703407Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGT1180.18116776442049345Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATATTTATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1100.16888520412079897Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCACACG1060.16274392397095175No Hit
ATATTTATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC970.14892604363379547Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC940.14432008352141004Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC880.1351081632966392Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC830.12743156310933013Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA780.11975496292202109Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC710.10900772265978843Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG710.10900772265978843Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)