FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001602400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65133
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG951114.602428876299264Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT927714.2431639875332Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGA38725.944759185052124Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCA37655.78047994104371Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGC34485.293783489168318Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG31594.850075998341855Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG17692.71598114626994Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCG9721.4923310764128783Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA9051.389464633902937Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAG8641.326516512367003Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAG8231.2635683908310686Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAG7721.1852670689205165Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAG7321.123854267422044Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG5480.8413553805290713Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTC3850.5910982144227964Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG2870.44063685075153913Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTC2700.4145364101146884Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT2610.4007185297775322Illumina Single End PCR Primer 1 (100% over 33bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA1990.30552868745489997Illumina Single End PCR Primer 1 (100% over 34bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1980.30399336741743815Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG1890.2901754870802819Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCC1720.2640750464434311Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT1700.2610044063685075No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA1310.20112692490749698Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT1200.18423840449541706Illumina Single End PCR Primer 1 (100% over 33bp)
TATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCC1170.17963244438303164Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG1110.17042052415826078Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT1090.16734988408333717Illumina Single End PCR Primer 1 (100% over 31bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGCT1060.16274392397095175Illumina Single End PCR Primer 1 (96% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGAG1040.1596732838960281Illumina Single End PCR Primer 1 (96% over 32bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1010.1550673237836427Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC980.1504613636712573Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA850.13050220318425376Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGT770.11821964288455929No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA710.10900772265978843Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG710.10900772265978843Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)