FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001602402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141903
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC2375716.74171793408173Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG2342116.504936470687724Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCG97666.882165986624667Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTT90616.385347737539022Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG83945.915308344432464Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCC83685.89698596928888Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG46433.271953376602327Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTG24571.7314644510686878Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA22481.5841807431837243Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTC22341.5743148488756404Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGC20821.4671994249593032Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTC20071.4143464197374263Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGC18611.311459236238839Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG12350.87031281932024Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCT8380.5905442450124381Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCT7710.5433288936808947Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT7380.5200735713832689Illumina Single End PCR Primer 1 (100% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGGAGGGAAAGAGTG5920.41718638788468176Illumina Single End PCR Primer 1 (96% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGAG5910.41648168114839007Illumina Single End PCR Primer 1 (96% over 32bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG5820.4101393205217648Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTC5120.36080984898134644Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGGAGGGAAAGAG4260.3002050696602609Illumina Single End PCR Primer 1 (96% over 30bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTC4190.29527212250621904Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT3830.2699026799997181No Hit
ATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT3800.26778855979084304Illumina Single End PCR Primer 1 (100% over 33bp)
TATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT3490.24594265096580056Illumina Single End PCR Primer 1 (100% over 31bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGGAGGGAAAG3490.24594265096580056Illumina Single End PCR Primer 1 (96% over 28bp)
TATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCT3340.2353720499214252Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCT3310.23325792971255013Illumina Single End PCR Primer 1 (96% over 33bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2550.17970021775438152No Hit
AACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2420.17053903018258953No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2350.16560608302854768Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTCTT1890.13318957315912983Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTGGGGAAAGAGTG1810.1275519192687963Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1800.1268472125325046Clontech SMART CDS Primer II A (100% over 26bp)
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1740.12261897211475443No Hit
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTG1730.12191426537846274Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGGAGGGAA1620.11416249127925414Illumina Single End PCR Primer 1 (96% over 26bp)
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGT1580.11134366433408736No Hit
ATATATATATATATATATAGATCGGAAGAGCGTTGTGTAGGGAAAGAGTG1560.10993425086150399Illumina Single End PCR Primer 1 (96% over 32bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTCTT1500.10570601044375383Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1480.10429659697117044Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCG1450.10218247676229536Illumina Single End PCR Primer 1 (96% over 33bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG1450.10218247676229536Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)