FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001602403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192332
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC3413017.74535698687686Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3277917.042925774182144Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAT127566.632281679595699TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA126196.561050683193645TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG120626.271447289062662Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTC106265.5248216625418545TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC70853.6837343759748764Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT35321.836407878044215Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGT32661.6981053594825615TruSeq Adapter, Index 7 (97% over 35bp)
TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAA28381.4755734875111786TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAA26801.3934238712226774TruSeq Adapter, Index 7 (97% over 35bp)
TATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATT25311.315953663456939TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATT24121.2540814841004098TruSeq Adapter, Index 7 (97% over 35bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21341.1095397541750722Clontech SMART CDS Primer II A (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA20331.0570263918640683Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG12710.6608364702701579Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG10750.5589293513299919Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG9880.5136950689432855Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA9390.48821828920824406Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC6350.3301582679949254No Hit
ATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGT5350.2781648399642285Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4930.2563276001913358No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG4930.2563276001913358Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4810.2500883888276522Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4750.24696878314581036Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCG4450.23137075473660132TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCG4310.22409167481230374TruSeq Adapter, Index 7 (97% over 35bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA4210.21889233200923403Clontech Universal Primer Mix Long (96% over 26bp)
ATATTTATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4060.21109331780462948Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATTTATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC3590.18665640663020194Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA3500.18197699810743923Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.18093712954682528No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA2990.15546034981178378Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.15390054697086286No Hit
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCACACG2840.14766133560717926No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.1331031757585841No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG2530.13154337291766321Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2480.12894370151612836Clontech SMART CDS Primer II A (100% over 21bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2470.12842376723582138Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC2440.12686396439490047Clontech Universal Primer Mix Long (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC2330.12114468731152382Clontech Universal Primer Mix Long (96% over 25bp)
ATATTTATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAT2080.1081463303038496TruSeq Adapter, Index 7 (97% over 35bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.10606659318262172No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG2040.10606659318262172Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2000.10398685606139382Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG1930.10034731609924506Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)