FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001602405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122978
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1570412.769763697571923Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1556612.65754850461058Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAT63185.1375042690562545TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA62125.051309990404787TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG59244.817121761615899Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTC53044.31296654686204TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC35712.9037714062677877Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT16681.3563401584023158Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16461.3384507798142757Clontech SMART CDS Primer II A (100% over 26bp)
ATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGT15311.2449381190131568TruSeq Adapter, Index 7 (97% over 35bp)
TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAA14531.1815121403828326TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAA13591.1050757045975703TruSeq Adapter, Index 7 (97% over 35bp)
TATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATT13531.1001967831644686TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATT12431.0107498902242678TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA8940.7269592935321765Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG6920.5627022719510807Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6800.5529444290848771Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG5480.4456081575566362Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5420.4407292361235343Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG3990.3244482753012734Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA3980.32363512172908976Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG3690.3000536681357641Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA3520.2862300574086422Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA2790.22686984663923626Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGT2540.20654100733464523Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCG2500.20328839304591065TruSeq Adapter, Index 7 (97% over 35bp)
TATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2470.20084893232935971Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCG2460.20003577875717607TruSeq Adapter, Index 7 (97% over 35bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC2460.20003577875717607Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC2310.18783847517442145No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG2230.1813332465969523Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG1940.15775179300362666Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA1880.15287287157052482Clontech SMART CDS Primer II A (96% over 26bp)
ATATTTATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1830.1488071037096066Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1820.14799395013742295Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC1740.14148872155995382Clontech Universal Primer Mix Long (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC1710.13904926084340288Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1580.12847826440501553Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA1440.11709411439444453Clontech Universal Primer Mix Long (96% over 25bp)
ATATTTATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1380.11221519296134269Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCT1380.11221519296134269Clontech SMART CDS Primer II A (96% over 25bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT1340.10896257867260811Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC1320.10733627152824082Clontech Universal Primer Mix Long (96% over 25bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1320.10733627152824082No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)