FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001602408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198678
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG2555012.860004630608321Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG2520212.684846837596513Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAG107335.402208598838321Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTC101975.132425331440824Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA88964.477596915612197Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG83524.203787032283393Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG48492.440632581362808Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA25731.2950603489062704Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCA25361.2764372502239805Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACG24141.215031357271565Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCA23711.1933882966407956Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT23301.1727518899928526Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG21181.0660465678132456Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT20081.010680598757789Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA15710.7907267035102025Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG15420.7761302207592184Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGT10600.533526610898036Illumina Single End PCR Primer 1 (96% over 33bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG9390.4726240449370338Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGT9100.4580275621860498Illumina Single End PCR Primer 1 (96% over 33bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA8540.4298412506669082Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT8440.4248079807527758Illumina Single End PCR Primer 1 (100% over 33bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG7630.3840384944483033Illumina Single End PCR Primer 1 (100% over 22bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA7600.3825285134740636Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG7080.35635550992057496Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT5530.27833982625152254No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC4890.24612689880107508Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC4830.24310693685259563Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA4510.22700047312737195Clontech Universal Primer Mix Long (96% over 25bp)
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA4420.22247053020465277Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT4390.220960549230413Illumina Single End PCR Primer 1 (100% over 33bp)
ATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTAC4220.2124039903763879Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT4190.21089400940214822Illumina Single End PCR Primer 1 (100% over 31bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG3920.1973041806339907Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC3780.1902576027542053Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC3610.1817010439001802Clontech Universal Primer Mix Long (96% over 25bp)
TATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTAC3600.18119771690876693Illumina Single End PCR Primer 1 (96% over 33bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC3540.17817775496028748Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCT3490.1756611200032213Clontech SMART CDS Primer II A (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3470.17465446602039483Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC3390.17062785008908887Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT3300.1660979071663697Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGT2680.13489163369874874No Hit
CTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTC2470.12432176687907066Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2460.12381843988765742Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT2420.12180513192200444Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2400.12079847793917797Clontech SMART CDS Primer II A (100% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC2370.11928849696493825Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAC2200.11073193811091313Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG2120.10670532217960721Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGAG2100.10569866819678073Illumina Single End PCR Primer 1 (96% over 32bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG2050.1031820332397145Clontech Universal Primer Mix Long (96% over 25bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT2040.10267870624830126Clontech Universal Primer Mix Long (96% over 26bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG2040.10267870624830126Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)