FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001602478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2585565
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA883043.415269003099903Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT760642.941871505841083Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG748332.8942610222523895Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG594062.2976022648821437Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGA472741.828381804363843Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAA402451.5565263298350651Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATT226420.8757080173965845Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG181200.7008139420204095Clontech SMART CDS Primer II A (96% over 26bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA165000.6381583909126245Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG162480.6284119718514135Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT128340.4963711993316741Clontech SMART CDS Primer II A (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA117560.45467818445871594Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAG114760.44384882994625935Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG113620.4394397356090448Illumina Single End PCR Primer 1 (100% over 30bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT109400.423118351308128Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG104850.40552065022538597Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA103400.39991259163857806Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC102190.39523276343855207Clontech Universal Primer Mix Long (96% over 25bp)
TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAG101560.3927961586732494Illumina Single End PCR Primer 1 (96% over 33bp)
CTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCT94280.36463983694086205Clontech SMART CDS Primer II A (96% over 25bp)
CTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTC92240.35674987865321506Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC90830.3512965251308708Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC90450.349826827018466Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA83790.3240684337852655Clontech Universal Primer Mix Long (96% over 25bp)
ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGAT73060.28256880024288694Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG72540.28055763440485926Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC69650.26938019349735937Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT69190.2676010852560272Clontech SMART CDS Primer II A (96% over 26bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG69160.26748505645767945Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG65110.25182116868073323Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG64610.2498873553749374Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC64610.2498873553749374Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA64020.24760545567409833Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGAT62470.24161063442613126Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC59280.22927290553515384Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC58970.2280739412855604Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG56180.2172832630392197Illumina Single End PCR Primer 1 (100% over 28bp)
ACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCG55650.21523342093507608Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG54580.211095060460673Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC52080.20142599393169386Clontech SMART CDS Primer II A (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT51900.2007298211416073Clontech Universal Primer Mix Long (96% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC51750.2001496771498686Clontech SMART CDS Primer II A (96% over 26bp)
TTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGAT49930.19311059671677178Clontech SMART CDS Primer II A (96% over 26bp)
ACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCT49570.19171825113659877Clontech SMART CDS Primer II A (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC48770.18862414984732542Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA43160.1669267645562962Clontech SMART CDS Primer II A (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA42200.16321384300916822Clontech SMART CDS Primer II A (96% over 26bp)
TATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTA41800.16166679236453155Illumina Single End PCR Primer 1 (96% over 33bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA41400.16011974171989488Clontech Universal Primer Mix Long (96% over 25bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG40870.1580698996157513Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTA39740.1536994815446527Illumina Single End PCR Primer 1 (96% over 33bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC39620.15323536635126173Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG38090.14731789763552647Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC37160.14372100488674625Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC35230.13625648552637432Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT33350.12898534749658197Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA32280.1248469870221789Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC31240.12082465534612356Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC30580.11827202178247309Clontech Universal Primer Mix Long (96% over 25bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA30530.1180786404518935Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30510.11800128791966166Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA30300.11718908633122742Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG29490.11405630877583815Clontech Universal Primer Mix Long (96% over 25bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG28690.11096220748656484Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA28500.11022735843036241Illumina Single End PCR Primer 1 (100% over 26bp)
AGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG28390.10980191950308733Clontech Universal Primer Mix Long (95% over 24bp)
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT28320.1095311856402759Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGTAAGCAGTGGTATCAACGCAGA26930.1041551846501635Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)